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KMID : 0895820040140020029
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Journal of Oriental Rehabilitation Medicine
2004 Volume.14 No. 2 p.29 ~ p.39
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Effects of Cheongyeolsaseup-tang (Qingrexieshi-tang ) in Experimental Model of Rheumatoid Arthritis
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Park Jin-Sang
Kwon Young-Dal
Shin Byung-Cheul
Lim Yang-Eui
Han Myeng-Gum
Lee Su-Kyung
Song Yuug-Sun
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Abstract
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Objective: This study was designed to understand the mechanism of action of Cheongyeolsaseup-tang(Qingrexieshi-tang) (CYSST) leading to control of mast cells on inflammatory condition like RA.
Methods: Viability of cells was evaluated by MTT colorimetric assay for 8 h incubation after stimulation of PMA plus A23187. HMC-1 cells (3×105 cells/㎖) were stimulated for 8 hour incubation with PMA plus A23187, we investigated the effect of CYSST on the production of cytokines by activated human mast cell line HMC-1.
Results: When CYSST (1 mg/ml) was added, the production of tumor necrosis factor (TNF)-α, interleukin (IL)-6 was significantly inhibited by 37% and 33.6%, respectively in phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated HMC-1 cells. RA is an aggressive inflammatory disease in which chemokines are thought to recruit leukocytes and induce angiogenesis. CYSST also inhibited by 48% the production of IL-8 in HMC-1 cells. In addition, CYSST inhibited the expression of Nuclear factor-κB(NF-κB) in HMC-1 cells.
Conclusions: When CYSST was added, the production of TNF-α, IL-6, IL-8 was significantly inhibited. These results imply that CYSST has a critical role on the treatment of the bone damage associated with rheumatoid arthritis.
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KEYWORD
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RA, Cheongyeolsaseup-tang(Qingrexieshi-tang), TNF-α, IL-6, IL-8, NF-κB
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